P-RGD naturally adsorbed on the oxidized titanium surface as a consequence of the bonding and dissociation mechanism of the phosphate functional group. The amount of P-RGD adsorbed at pH 1.9 was approximately 3.6 times as much as that at pH 3.0 ( P < 0.05). The oxidized titanium surface was etched when exposed to H 3PO 4 solutions with a pH of 2.0 or below. An SPR instrument for ‘single-spot’ analysis was used for nanometer-scale detection of biomolecular adsorption using a He–Ne laser light according to Knoll’s method.
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